Photobiomodulation therapy improves human dental pulp stem cell viability and migration in vitro associated to upregulation of histone acetylation.
Lasers Med Sci
; 35(3): 741-749, 2020 Apr.
Article
en En
| MEDLINE
| ID: mdl-32095920
ABSTRACT
This in vitro study evaluated the role of photobiomodulation therapy (PBMT) on viability and migration of human dental pulp stem cells (hDPSCs) and its association to epigenetic mechanisms such as histone acetylation. The hDPSCs were characterized and assigned into control and PBMT groups. For the PBMT, five laser irradiations at 6-h intervals were performed using a continuous-wave InGaAlP diode laser. Viability (MTT), migration (scratch), and histone acetylation H3 (H3K9ac immunofluorescence) were evaluated immediately after the last irradiation. PBMT significantly increased the viability (P = 0.004). Also, PBMT group showed significantly increased migration of cells in the wound compared to the control in 6 h (P = 0.002), 12 h (P = 0.014) and 18 h (P = 0.083) being faster than the control, which only finished the process at 24 h. PBMT induced epigenetic modifications in hDPSC due to increased histone acetylation (P = 0.001). PBMT increased viability and migration of hDPSCs, which are related with the upregulation of histone acetylation and could be considered a promising adjuvant therapy for regenerative endodontic treatment.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
Histonas
/
Regulación hacia Arriba
/
Movimiento Celular
/
Terapia por Luz de Baja Intensidad
/
Pulpa Dental
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Lasers Med Sci
Asunto de la revista:
BIOTECNOLOGIA
/
RADIOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Brasil