A lysosome-targeted near-infrared fluorescent probe for imaging endogenous cysteine (Cys) in living cells.
J Mater Chem B
; 8(11): 2269-2274, 2020 03 18.
Article
en En
| MEDLINE
| ID: mdl-32100785
ABSTRACT
Cysteine (Cys) is one of the most important essential biothiols in lysosomes. Highly selective probes for specific detection and imaging of lysosomal Cys over other biological thiols are rare. Herein, we developed a lysosome-targeted near-infrared fluorescent probe SHCy-C based on a novel NIR-emitting thioxanthene-indolium dye. Due to the turn-on fluorescence response elicited by the intramolecular charge transfer (ICT) processes before and after the reaction with Cys, probe SHCy-C exhibits high selectivity and sensitivity (16 nM) for the detection of Cys. More importantly, probe SHCy-C is found to precisely target lysosomes and achieves the "turn-on" detection and imaging of endogenous Cys in lysosomes.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos de Sulfhidrilo
/
Carbocianinas
/
Cisteína
/
Colorantes Fluorescentes
/
Lisosomas
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Mater Chem B
Año:
2020
Tipo del documento:
Article
País de afiliación:
China