Genotypic and phenotypic analysis of biofilm formation Staphylococcus epidermidis isolates from clinical specimens.
BMC Res Notes
; 13(1): 114, 2020 Feb 27.
Article
en En
| MEDLINE
| ID: mdl-32103775
ABSTRACT
OBJECTIVES:
Staphylococcus epidermidis is the primary causative agent of infections associated with indwelling biomaterials. Antibiotic susceptibility patterns, Biofilm formation capability, and screening of responsible genes in biofilm formation procedure in clinical isolates (icaA, icaB, icaC, icaD, sdrG, and atlE) were assigned as the main objectives in this study. The clinical samples were analyzed via standard biochemical assays for identifying different bacteria which were confirmed using the multiplex colony PCR method. Subsequently, biofilm-formation capability, antibiotic susceptibility testing, and the frequency of genes responsible for biofilm formation in the confirmed strains were checked.RESULTS:
Out of 183 clinical specimens 54 S. epidermidis isolates were detected by targeting a housekeeping gene (sesc) taking advantage of the PCR procedure. All of the strains were Biofilm forming producers. The in vitro biofilm formation assays determined that 45 (83.33%), 5 (9.26%), 4 (7.41%) were strong, moderate, and weak biofilm former strains respectively. Among the isolated strains, the specific frequencies of the biofilm-forming genes were specified to be (98%) for sdrG, (84%) for atlE, (80%) for icaC, and (70%) for icaD. Cefamandole and Amikacin are the most effective antibiotics in isolated strains. All strains were ascertained to be methicillin and amoxicillin/clavulanic acid resistant.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Staphylococcus epidermidis
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Proteínas Bacterianas
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Biopelículas
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Farmacorresistencia Bacteriana
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Antibacterianos
Límite:
Humans
Idioma:
En
Revista:
BMC Res Notes
Año:
2020
Tipo del documento:
Article
País de afiliación:
Irán