HLA Typing from RNA Sequencing and Applications to Cancer.
Methods Mol Biol
; 2120: 71-92, 2020.
Article
en En
| MEDLINE
| ID: mdl-32124312
ABSTRACT
The human leukocyte antigen (HLA) complex is necessary for antigen presentation and regulates both innate and adaptive immune responses. In the context of cancer and treatment therapies, the HLA locus plays a critical role in tumor recognition and tolerance mechanisms. In silico HLA class I and class II typing, as well as expression quantification from next-generation RNA sequencing, can therefore have great potential clinical applications. However, HLA typing from short-read data is a challenging task given the high polymorphism and homology at the HLA locus. In this chapter, we present our highly accurate HLA typing solution, arcasHLA. We provide a detailed outline for practitioners using our protocol to perform HLA typing and demonstrate the applicability of arcasHLA in several clinical samples from tumors.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Prueba de Histocompatibilidad
/
Análisis de Secuencia de ARN
/
Antígenos HLA
/
Neoplasias
Tipo de estudio:
Guideline
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos