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Hydrogel cross-linking-programmed release of nitric oxide regulates source-dependent angiogenic behaviors of human mesenchymal stem cell.
Kang, Mi-Lan; Kim, Hye-Seon; You, Jin; Choi, Young Sik; Kwon, Byeong-Ju; Park, Chan Hee; Baek, Wooyeol; Kim, Min Sup; Lee, Yong Jae; Im, Gun-Il; Yoon, Jeong-Kee; Lee, Jung Bok; Sung, Hak-Joon.
Afiliación
  • Kang ML; Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
  • Kim HS; TMD LAB Co., Ltd., Seoul 03722, Republic of Korea.
  • You J; Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
  • Choi YS; Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
  • Kwon BJ; Department of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
  • Park CH; Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
  • Baek W; Metareceptome Research Center, College of Pharmacy, Chung-Ang University, Seoul 06911, Republic of Korea.
  • Kim MS; Institute for Human Tissue Restoration, Department of Plastic & Reconstructive Surgery, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
  • Lee YJ; Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
  • Im GI; Department of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
  • Yoon JK; Department of Orthopedics, Dongguk University Ilsan Hospital, Goyang 10326, Republic of Korea.
  • Lee JB; Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
  • Sung HJ; Department of Medical Engineering, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
Sci Adv ; 6(9): eaay5413, 2020 02.
Article en En | MEDLINE | ID: mdl-32133403
Angiogenesis is stimulated by nitric oxide (NO) production in endothelial cells (ECs). Although proangiogenic actions of human mesenchymal stem cells (hMSCs) have been extensively studied, the mechanistic role of NO in this action remains obscure. Here, we used a gelatin hydrogel that releases NO upon crosslinking by a transglutaminase reaction ("NO gel"). Then, the source-specific behaviors of bone marrow versus adipose tissue-derived hMSCs (BMSCs versus ADSCs) were monitored in the NO gels. NO inhibition resulted in significant decreases in their angiogenic activities. The NO gel induced pericyte-like characteristics in BMSCs in contrast to EC differentiation in ADSCs, as evidenced by tube stabilization versus tube formation, 3D colocalization versus 2D coformation with EC tube networks, pericyte-like wound healing versus EC-like vasculogenesis in gel plugs, and pericyte versus EC marker production. These results provide previously unidentified insights into the effects of NO in regulating hMSC source-specific angiogenic mechanisms and their therapeutic applications.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de la Médula Ósea / Tejido Adiposo / Neovascularización Fisiológica / Hidrogeles / Células Madre Mesenquimatosas / Óxido Nítrico Límite: Humans Idioma: En Revista: Sci Adv Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de la Médula Ósea / Tejido Adiposo / Neovascularización Fisiológica / Hidrogeles / Células Madre Mesenquimatosas / Óxido Nítrico Límite: Humans Idioma: En Revista: Sci Adv Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos