Qualitative and quantitative determination of pheromone secretion in female gametes of Ectocarpus siliculosus (Phaeophyceae).
Biol Chem Hoppe Seyler
; 369(8): 647-53, 1988 Aug.
Article
en En
| MEDLINE
| ID: mdl-3214549
ABSTRACT
Pheromone secretion in living female gametes of Ectocarpus siliculosus was examined by closed-loop extraction and gas chromatography. The pheromone (ectocarpene) recovery efficiency was calibrated with synthetic ectocarpene and was found to depend strongly on the volume of the closed-loop system. Maximum extraction efficiency of ectocarpene (17.4%) was obtained with a small-volume system. In addition to the main compound ectocarpene, the pheromone bouquet of E. siliculosus contained minor amounts of hormosirene, multifidene and dictyotene, which until now are known only as sperm-attractants in other brown algae. The pheromone bouquet was identical in 4 clones of different geographic origins. Settled female gametes of Ectocarpus siliculosus continued to secrete pheromone and to attract male gametes for up to 7 h after their release with an ectocarpene secretion rate of 1 x 10(5) molecules s-1 cell-1.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Feromonas
/
Cicloheptanos
/
Phaeophyceae
/
Eucariontes
Tipo de estudio:
Qualitative_research
Idioma:
En
Revista:
Biol Chem Hoppe Seyler
Asunto de la revista:
BIOQUIMICA
Año:
1988
Tipo del documento:
Article