Expression profiles of miRNAs in giant cell tumor of bone showed miR-187-5p and miR-1323 can regulate biological functions through inhibiting FRS2.
Cancer Med
; 9(9): 3163-3173, 2020 05.
Article
en En
| MEDLINE
| ID: mdl-32154662
ABSTRACT
BACKGROUND:
Giant cell tumor of bone (GCTB) is considered to be a kind of borderline tumor, which has a tendency to recur and translocate. MicroRNAs are one type of small noncoding RNA, which can inhibit the translation of targeted mRNA through RNA-induced silencing complex.METHODS:
Microarray was conducted on three groups of tumor tissues and normal tissues from patients with GCTB, and results showed different expression profiles of miRNAs with Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis. The functions of miR-187-5p and miR-1323, which were highly expressed in GCTB, were examined by 5-ethynyl-2'-deoxyuridine (EDU), transwell, and CCK8 assays. RNAhybrid et al. (RNA prediction softwares) predicted that the two microRNAs targeted fibroblast growth factor receptor substrate 2 (FRS2), which was verified by luciferase assay and rescue experiments.RESULTS:
miR-187-5p and miR-1323 were highly expressed in tumor tissues. They can jointly regulate the biological functions of GCTB in vitro. Luciferase assay confirmed that the two microRNAs can bind to the 3' untranslated regions (UTR) of mRNA of FRS2. And, rescue experiments verified the relationships between the two microRNAs and FRS2.CONCLUSION:
There were some different-expressed microRNAs between GCTB and normal tissues. miR-187-5p and miR-1323 can regulate the biological functions of GCTB through influencing the expression of FRS2.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Óseas
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Biomarcadores de Tumor
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Regulación Neoplásica de la Expresión Génica
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Tumor Óseo de Células Gigantes
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MicroARNs
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Proteínas Adaptadoras Transductoras de Señales
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Proteínas de la Membrana
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Cancer Med
Año:
2020
Tipo del documento:
Article
País de afiliación:
China