Experimental concepts for linking the biological activities of antimicrobial peptides to their molecular modes of action.
Biochim Biophys Acta Biomembr
; 1862(8): 183275, 2020 08 01.
Article
en En
| MEDLINE
| ID: mdl-32173291
The search for novel compounds to combat multi-resistant bacterial infections includes exploring the potency of antimicrobial peptides and derivatives thereof. Complementary to high-throughput screening techniques, biophysical and biochemical studies of the biological activity of these compounds enable deep insight, which can be exploited in designing antimicrobial peptides with improved efficacy. This approach requires the combination of several techniques to study the effect of such peptides on both bacterial cells and simple mimics of their cell envelope, such as lipid-only vesicles. These efforts carry the challenge of bridging results across techniques and sample systems, including the proper choice of membrane mimics. This review describes some important concepts toward the development of potent antimicrobial peptides and how they translate to frequently applied experimental techniques, along with an outline of the biophysics pertaining to the killing mechanism of antimicrobial peptides.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Infecciones Bacterianas
/
Membrana Celular
/
Péptidos Catiónicos Antimicrobianos
/
Antibacterianos
Límite:
Humans
Idioma:
En
Revista:
Biochim Biophys Acta Biomembr
Año:
2020
Tipo del documento:
Article
Pais de publicación:
Países Bajos