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Down-regulation of lncRNA NEAT1 regulated by miR-194-5p/DNMT3A facilitates acute myeloid leukemia.
Duan, Ming-Yue; Li, Ming; Tian, Hui; Tang, Gen; Yang, Yu-Cong; Peng, Nian-Cai.
Afiliación
  • Duan MY; State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, PR China.
  • Li M; State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, PR China.
  • Tian H; State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, PR China.
  • Tang G; Institute of Pediatrics, Shenzhen Children's Hospital, Shenzhen 528038, PR China.
  • Yang YC; Clinical Laboratory, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, PR China.
  • Peng NC; State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710054, PR China. Electronic address: ncpeng@mail.xjtu.edu.cn.
Blood Cells Mol Dis ; 82: 102417, 2020 05.
Article en En | MEDLINE | ID: mdl-32179410
OBJECTIVE: miR-194-5p and NEAT1 have been reported to be associated with multiple malignancies, but their roles in acute myeloid leukemia (AML) remains not fully understood. METHODS: Bone marrow samples were collected for monocyte separation. qRT-PCR assay was performed to investigate the expression patterns of NEAT1 and miR-194-5p in AML. CCK-8, soft agar colony formation, flow cytometry and transwell assays were employed to explore the biological functions of NEAT1 or miR-194-5p. Methylation PCR was performed to monitor the methylation of NEAT1. Luciferase reporter assay was subjected to verify the relationship between miR-194-5p and DNMT3A. Immunofluorescence and western blotting were performed to detect the alterations of protein expression. RESULTS: NEAT1 and miR-194-5p were both down-regulated in AML. Overexpression of either NEAT1 or miR-194-5p repressed proliferation, induced apoptosis and restrained migration and invasion of AML cells. There was a negative correlation between NEAT1 and DNMT3A in AML. Knockdown of DNMT3A dramatically decreased the methylation of NEAT1. Moreover, DNMT3A was identified as a downstream target of miR-194-5p. Furthermore, down-regulation of DNMT3A rescued the impacts on the malignant phenotypes of NEAT1 inhibition by miR-194-5p inhibitor. CONCLUSION: Altogether, down-regulation of NEAT1 mediated by miR-194-5p/DNMT3A axis promotes AML progression, which might provide therapeutic targets in AML treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Neoplásico / Leucemia Mieloide Aguda / Regulación hacia Abajo / Regulación Leucémica de la Expresión Génica / MicroARNs / ADN (Citosina-5-)-Metiltransferasas / ARN Largo no Codificante / Proteínas de Neoplasias Límite: Female / Humans / Male Idioma: En Revista: Blood Cells Mol Dis Asunto de la revista: HEMATOLOGIA Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Neoplásico / Leucemia Mieloide Aguda / Regulación hacia Abajo / Regulación Leucémica de la Expresión Génica / MicroARNs / ADN (Citosina-5-)-Metiltransferasas / ARN Largo no Codificante / Proteínas de Neoplasias Límite: Female / Humans / Male Idioma: En Revista: Blood Cells Mol Dis Asunto de la revista: HEMATOLOGIA Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos