Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies.
Nat Methods
; 17(5): 471-479, 2020 05.
Article
en En
| MEDLINE
| ID: mdl-32203383
ABSTRACT
Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have emerged as a biotechnological tool and therapeutic. The discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPR (Acr) proteins, enables the development of more controllable and precise CRISPR-Cas tools. Here we discuss applications of Acr proteins for post-translational control of CRISPR-Cas systems in prokaryotic and mammalian cells, organisms and ecosystems.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Regulación de la Expresión Génica
/
Inhibidores Enzimáticos
/
Proteínas Asociadas a CRISPR
/
Sistemas CRISPR-Cas
/
Edición Génica
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos