Identification and comprehensive analysis of the CLV3/ESR-related (CLE) gene family in Brassica napus L.
Plant Biol (Stuttg)
; 22(4): 709-721, 2020 Jul.
Article
en En
| MEDLINE
| ID: mdl-32223006
ABSTRACT
The CLE (CLAVATA3/ESR) gene family, encoding a group of small secretory peptides, plays important roles in cell-to-cell communication, thereby controlling a broad spectrum of development processes. The CLE family has been systematically characterized in some plants, but not in Brassica napus. In the present study, 116 BnCLE genes were identified in the B. napus genome, including seven unannotated, six incorrectly predicted and five multi-CLE domain-encoding genes. These BnCLE members were separated into seven distinct groups based on phylogenetic analysis, which might facilitate the functional characterization of the peptides. Further characterization of CLE pre-propeptides revealed 31 unique CLE peptides from 45 BnCLE genes, which may give rise to distinct roles of BnCLE and expansion of the gene family. The biological activity of these unique CLE dodecamer peptides was tested further through in vitro peptide assays. Variations in several important residues were identified as key contributors to the functional differentiation of BnCLE and expansion of the gene family in B. napus. Expression profile analysis helped to characterize possible functional redundancy and sub-functionalization among the BnCLE members. This study presents a comprehensive overview of the CLE gene family in B. napus and provides a foundation for future evolutionary and functional studies.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genes de Plantas
/
Regulación de la Expresión Génica de las Plantas
/
Brassica napus
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Plant Biol (Stuttg)
Asunto de la revista:
BOTANICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
China