An endoxylanase rapidly hydrolyzes xylan into major product xylobiose via transglycosylation of xylose to xylotriose or xylotetraose.
Carbohydr Polym
; 237: 116121, 2020 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-32241400
ABSTRACT
Here, we proposed an effective strategy to enhance a novel endoxylanase (Taxy11) activity and elucidated an efficient catalysis mechanism to produce xylooligosaccharides (XOSs). Codon optimization and recruitment of natural propeptide in Pichia pastoris resulted in achievement of Taxy11 activity to 1405.65⯱â¯51.24 U/mL. Analysis of action mode reveals that Taxy11 requires at least three xylose (xylotriose) residues for hydrolysis to yield xylobiose. Results of site-directed mutagenesis indicate that residues Glu119, Glu210, and Asp53 of Taxy11 are key catalytic sites, while Asp203 plays an auxiliary role. The novel mechanism whereby Taxy11 catalyzes conversion of xylan or XOSs into major product xylobiose involves transglycosylation of xylose to xylotriose or xylotetraose as substrate, to form xylotetraose or xylopentaose intermediate, respectively. Taxy11 displayed highly hydrolytic activity toward corncob xylan, producing 50.44 % of xylobiose within 0.5â¯h. This work provides a cost-effective and sustainable way to produce value-added biomolecules XOSs (xylobiose-enriched) from agricultural waste.
Palabras clave
Action mode; Biomass conversion; Catalysis mechanism; Improved expression; Sodium carboxymethyl cellulose (PubChem CID: 23706213); Xylanolytic enzyme; Xylobiose; Xylobiose (PubChem CID: 126963631); Xylopentaose (PubChem CID: 10146542); Xylose (PubChem CID: 644160); Xylotetraose (PubChem CID: 101601989); Xylotriose (PubChem CID: 12444993); pNP-α-l-arabinofuranoside (PubChem CID: 151437); pNP-ß-d- cellobioside (PubChem CID: 165125); pNP-ß-d-glucopyranoside (PubChem CID: 92930); pNP-ß-d-xylopyranoside (PubChem CID: 91509)
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Xilanos
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Endo-1,4-beta Xilanasas
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Xilano Endo-1,3-beta-Xilosidasa
/
Disacáridos
Idioma:
En
Revista:
Carbohydr Polym
Año:
2020
Tipo del documento:
Article