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Effect of scopoletin on phagocytic activity of U937-derived human macrophages: Insights from transcriptomic analysis.
Alkorashy, Amgad I; Doghish, Ahmed S; Abulsoud, Ahmed I; Ewees, Mohamed G; Abdelghany, Tamer M; Elshafey, Mostafa M; Elkhatib, Walid F.
Afiliación
  • Alkorashy AI; Department of Biochemistry, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo 11651, Egypt.
  • Doghish AS; Department of Biochemistry, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo 11651, Egypt; Department of Biochemistry, Faculty of Pharmacy, Badr University in Cairo (BUC), Badr City, Cairo 11829, Egypt. Electronic address: ahmed_doghish@azhar.edu.eg.
  • Abulsoud AI; Department of Biochemistry, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo 11651, Egypt; Department of Biochemistry and Biotechnology, Faculty of Pharmacy, Heliopolis University, Cairo 11785, Egypt.
  • Ewees MG; Department of Pharmacology and Toxicology, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo 11651, Egypt; Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
  • Abdelghany TM; Department of Pharmacology and Toxicology, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo 11651, Egypt; Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
  • Elshafey MM; Department of Biochemistry, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo 11651, Egypt.
  • Elkhatib WF; Department of Microbiology and Immunology, Faculty of Pharmacy, Badr University in Cairo (BUC), Badr City, Cairo 11829, Egypt; Microbiology and Immunology Department, Faculty of Pharmacy, Ain Shams University, African Union Organization St., Abbassia, Cairo 11566, Egypt. Electronic address: walid-el
Genomics ; 112(5): 3518-3524, 2020 09.
Article en En | MEDLINE | ID: mdl-32243896
ABSTRACT
Scopoletin is a botanical coumarin. Notably, scopoletin effect on phagocytic activity has not been addressed on transcriptomic level. Accordingly, this study investigated the effect of scopoletin on phagocytosis-linked gene transcription. Whole phagocytosis transcriptional profiling of stimulated U937-derived macrophages (SUDMs) in response to scopoletin as compared to non-treated SUDMs was studied. Regarding scopoletin effect on 92 phagocytosis-linked genes, 12 of them were significantly affected (p-value < .05). Seven genes were downregulated (CDC42, FCGR1A/FCGR1C, ITGA9, ITGB3, PLCE1, RHOD & RND3) and five were upregulated (DIRAS3, ITGA1, PIK3CA, PIK3R3 & PLCD1). Moreover, scopoletin enhanced phagocytic activity of SUDMs. The current results highlighted the potential use of scopoletin as immunity booster and as an adjuvant remedy in management of some autoimmune reactions. To the best of our knowledge, this is the first report that unravels the effect of scopoletin on phagocytosis via transcriptomic analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fagocitosis / Escopoletina / Transcriptoma / Macrófagos Límite: Humans Idioma: En Revista: Genomics Asunto de la revista: GENETICA Año: 2020 Tipo del documento: Article País de afiliación: Egipto
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fagocitosis / Escopoletina / Transcriptoma / Macrófagos Límite: Humans Idioma: En Revista: Genomics Asunto de la revista: GENETICA Año: 2020 Tipo del documento: Article País de afiliación: Egipto