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Genetic tool development in marine protists: emerging model organisms for experimental cell biology.
Faktorová, Drahomíra; Nisbet, R Ellen R; Fernández Robledo, José A; Casacuberta, Elena; Sudek, Lisa; Allen, Andrew E; Ares, Manuel; Aresté, Cristina; Balestreri, Cecilia; Barbrook, Adrian C; Beardslee, Patrick; Bender, Sara; Booth, David S; Bouget, François-Yves; Bowler, Chris; Breglia, Susana A; Brownlee, Colin; Burger, Gertraud; Cerutti, Heriberto; Cesaroni, Rachele; Chiurillo, Miguel A; Clemente, Thomas; Coles, Duncan B; Collier, Jackie L; Cooney, Elizabeth C; Coyne, Kathryn; Docampo, Roberto; Dupont, Christopher L; Edgcomb, Virginia; Einarsson, Elin; Elustondo, Pía A; Federici, Fernan; Freire-Beneitez, Veronica; Freyria, Nastasia J; Fukuda, Kodai; García, Paulo A; Girguis, Peter R; Gomaa, Fatma; Gornik, Sebastian G; Guo, Jian; Hampl, Vladimír; Hanawa, Yutaka; Haro-Contreras, Esteban R; Hehenberger, Elisabeth; Highfield, Andrea; Hirakawa, Yoshihisa; Hopes, Amanda; Howe, Christopher J; Hu, Ian; Ibañez, Jorge.
Afiliación
  • Faktorová D; Institute of Parasitology, Biology Centre, Czech Academy of Sciences and Faculty of Sciences, University of South Bohemia, Ceské Budejovice, Czech Republic. dranov@paru.cas.cz.
  • Nisbet RER; Department of Biochemistry, University of Cambridge, Cambridge, UK.
  • Fernández Robledo JA; School of Biosciences, University of Nottingham, Sutton Bonington, UK.
  • Casacuberta E; Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, USA.
  • Sudek L; Institut de Biologia Evolutiva, CSIC-Universitat Pompeu Fabra, Barcelona, Spain.
  • Allen AE; Monterey Bay Aquarium Research Institute, Moss Landing, CA, USA.
  • Ares M; Integrative Oceanography Division, Scripps Institution of Oceanography, University of California, San Diego, CA, USA.
  • Aresté C; Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, CA, USA.
  • Balestreri C; Molecular, Cell and Developmental Biology, University of California, Santa Cruz, CA, USA.
  • Barbrook AC; Institut de Biologia Evolutiva, CSIC-Universitat Pompeu Fabra, Barcelona, Spain.
  • Beardslee P; The Marine Biological Association, Plymouth and School of Ocean and Earth Sciences, University of Southampton, Southampton, UK.
  • Bender S; Department of Biochemistry, University of Cambridge, Cambridge, UK.
  • Booth DS; School of Biological Sciences, University of Nebraska, Lincoln, NE, USA.
  • Bouget FY; Gordon and Betty Moore Foundation, Palo Alto, CA, USA.
  • Bowler C; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
  • Breglia SA; Sorbonne Université, CNRS UMR7621, Observatoire Océanologique, Banyuls sur Mer, France.
  • Brownlee C; Institut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, Université PSL, Paris, France.
  • Burger G; Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, Nova Scotia, Canada.
  • Cerutti H; The Marine Biological Association, Plymouth and School of Ocean and Earth Sciences, University of Southampton, Southampton, UK.
  • Cesaroni R; Department of Biochemistry and Robert-Cedergren Centre for Bioinformatics and Genomics, Université de Montréal, Montreal, Quebec, Canada.
  • Chiurillo MA; School of Biological Sciences, University of Nebraska, Lincoln, NE, USA.
  • Clemente T; Institute of Cell Biology, University of Bern, Bern, Switzerland.
  • Coles DB; Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, USA.
  • Collier JL; School of Biological Sciences, University of Nebraska, Lincoln, NE, USA.
  • Cooney EC; Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, USA.
  • Coyne K; School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY, USA.
  • Docampo R; Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada.
  • Dupont CL; University of Delaware College of Earth, Ocean and Environment, Lewes, DE, USA.
  • Edgcomb V; Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, USA.
  • Einarsson E; Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, CA, USA.
  • Elustondo PA; Woods Hole Oceanographic Institution, Woods Hole, MA, USA.
  • Federici F; Department of Biochemistry, University of Cambridge, Cambridge, UK.
  • Freire-Beneitez V; Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, Nova Scotia, Canada.
  • Freyria NJ; AGADA Biosciences Inc., Halifax, Nova Scotia, Canada.
  • Fukuda K; Facultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium Institute for Integrative Biology (iBio), Santiago de Chile, Chile.
  • García PA; School of Biosciences, University of Kent, Canterbury, Kent, UK.
  • Girguis PR; Laboratory of Molecular and Evolutionary Parasitology, University of Kent, Kent, UK.
  • Gomaa F; Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, USA.
  • Gornik SG; Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan.
  • Guo J; Department of Mechanical Engineering, Massachusetts Institute of Technology, Boston, MA, USA.
  • Hampl V; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.
  • Hanawa Y; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.
  • Haro-Contreras ER; Centre for Organismal Studies, University of Heidelberg, Heidelberg, Germany.
  • Hehenberger E; Monterey Bay Aquarium Research Institute, Moss Landing, CA, USA.
  • Highfield A; Molecular, Cell and Developmental Biology, University of California, Santa Cruz, CA, USA.
  • Hirakawa Y; Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic.
  • Hopes A; Faculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan.
  • Howe CJ; Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, Nova Scotia, Canada.
  • Hu I; Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada.
  • Ibañez J; The Marine Biological Association, Plymouth and School of Ocean and Earth Sciences, University of Southampton, Southampton, UK.
Nat Methods ; 17(5): 481-494, 2020 05.
Article en En | MEDLINE | ID: mdl-32251396
ABSTRACT
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transformación Genética / ADN / Proteínas Fluorescentes Verdes / Eucariontes / Biología Marina / Modelos Biológicos Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2020 Tipo del documento: Article País de afiliación: República Checa
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transformación Genética / ADN / Proteínas Fluorescentes Verdes / Eucariontes / Biología Marina / Modelos Biológicos Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2020 Tipo del documento: Article País de afiliación: República Checa