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Modulation of Wnt and Activin/Nodal supports efficient derivation, cloning and suspension expansion of human pluripotent stem cells.
Ai, Zongyong; Niu, Baohua; Duan, Kui; Si, Chenyang; Wang, Sile; Xiang, Lifeng; Zhu, Xiaoqing; Zhu, Qingyuan; Feng, Chun; Yin, Yu; Zhao, Shumei; Kong, Ruize; Ji, Weizhi; Li, Tianqing.
Afiliación
  • Ai Z; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; Yunnan Provincial Academy of Science and Technology, Kunming, Yunnan, 650500, China; XiAn ChaoYue Stem Cell Co., Ltd, No. 5
  • Niu B; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China.
  • Duan K; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; Yunnan Provincial Academy of Science and Technology, Kunming, Yunnan, 650500, China.
  • Si C; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; Yunnan Provincial Academy of Science and Technology, Kunming, Yunnan, 650500, China.
  • Wang S; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; Yunnan Provincial Academy of Science and Technology, Kunming, Yunnan, 650500, China.
  • Xiang L; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; The First People's Hospital of Yunnan Province, No.157, Jinbi Road, Xishan District, Kunming, Yunnan, 650034, China.
  • Zhu X; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; Yunnan Provincial Academy of Science and Technology, Kunming, Yunnan, 650500, China; XiAn ChaoYue Stem Cell Co., Ltd, No. 5
  • Zhu Q; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China.
  • Feng C; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; Yunnan Provincial Academy of Science and Technology, Kunming, Yunnan, 650500, China; XiAn ChaoYue Stem Cell Co., Ltd, No. 5
  • Yin Y; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; Yunnan Provincial Academy of Science and Technology, Kunming, Yunnan, 650500, China.
  • Zhao S; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; Yunnan Provincial Academy of Science and Technology, Kunming, Yunnan, 650500, China.
  • Kong R; The First People's Hospital of Yunnan Province, No.157, Jinbi Road, Xishan District, Kunming, Yunnan, 650034, China.
  • Ji W; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; Yunnan Provincial Academy of Science and Technology, Kunming, Yunnan, 650500, China.
  • Li T; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, 650500, China; Yunnan Provincial Academy of Science and Technology, Kunming, Yunnan, 650500, China; XiAn ChaoYue Stem Cell Co., Ltd, No. 5
Biomaterials ; 249: 120015, 2020 08.
Article en En | MEDLINE | ID: mdl-32311594
ABSTRACT
Various culture systems have been used to derive and maintain human pluripotent stem cells (hPSCs), but they are inefficient in sustaining cloning and suspension expansion of hPSCs. Through systematically modulating Wnt and Activin/Nodal signaling, we developed a defined medium (termed AIC), which enables efficient cloning and long-term expansion of hPSCs (AIC-hPSCs) through single-cell passage on feeders, matrix or in suspension (25-fold expansion in 4 days) and maintains genomic stability of hPSCs over extensive expansion. Moreover, the AIC medium supports efficient derivation of hPSCs from blastocysts or somatic cells under feeder-free conditions. Compared to conventional hPSCs, AIC-hPSCs have similar gene expression profiles but down-regulated differentiation genes and display higher metabolic activity. Additionally, the AIC medium shows a good compatibility for different hPSC lines under various culture conditions. Our study provides a robust culture system for derivation, cloning and suspension expansion of high-quality hPSCs that benefits GMP production and processing of therapeutic hPSC products.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activinas / Células Madre Pluripotentes Límite: Humans Idioma: En Revista: Biomaterials Año: 2020 Tipo del documento: Article
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activinas / Células Madre Pluripotentes Límite: Humans Idioma: En Revista: Biomaterials Año: 2020 Tipo del documento: Article