microRNA-1236 promotes chondrocyte apoptosis in osteoarthritis via direct suppression of PIK3R3.
Life Sci
; 253: 117694, 2020 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-32325132
ABSTRACT
AIMS:
Chondrocyte degeneration is the main cause of osteoarthritis (OA) and increased evidence suggests that miRNAs could have vital roles in the pathology of various cartilage illnesses. miR-1236 has been found to contribute to inflammation in diseases such as pneumonia. However, the exact role of miR-1236 in OA is poorly understood. MATERIALS ANDMETHODS:
H&E staining and saffron fixation experiments were employed to determine OA tissues. qRT-PCR and immunohistochemistry were used to detect the expression levels of miR-1236 and PIK3R3. Western blot was performed to detect the expression levels of proteins. Luciferase reporter assays were utilized to investigate the interaction between miR-1236 and PIK3R3. Cell counting assays and AO/EB were used to quantify cell growth and apoptosis. KEYFINDINGS:
miR-1236 was up-regulated in OA knee cartilage compared to normal cartilage. Up-regulated expression of miR-1236 suppressed cell proliferation as well as induced apoptosis in chondrocytes. Bioinformatics identified PIK3R3 as a target of miR-1236. Co-transfection with miR-1236 and PIK3R3 could reverse cell apoptosis induced by the miR-1236 mimic.SIGNIFICANCE:
These data enhance our understanding on the role of miR-1236 in OA and identifies miR-1236 as a potential biomarker or possible treatment target within OA.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Apoptosis
/
Condrocitos
/
Fosfatidilinositol 3-Quinasas
/
Osteoartritis de la Rodilla
/
MicroARNs
Tipo de estudio:
Prognostic_studies
Límite:
Female
/
Humans
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Male
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Middle aged
Idioma:
En
Revista:
Life Sci
Año:
2020
Tipo del documento:
Article
País de afiliación:
China