Differential requirement for Bub1 and Bub3 in regulation of meiotic versus mitotic chromosome segregation.
J Cell Biol
; 219(4)2020 04 06.
Article
en En
| MEDLINE
| ID: mdl-32328625
ABSTRACT
Accurate chromosome segregation depends on the proper attachment of kinetochores to spindle microtubules before anaphase onset. The Ipl1/Aurora B kinase corrects improper attachments by phosphorylating kinetochore components and so releasing aberrant kinetochore-microtubule interactions. The localization of Ipl1 to kinetochores in budding yeast depends upon multiple pathways, including the Bub1-Bub3 pathway. We show here that in meiosis, Bub3 is crucial for correction of attachment errors. Depletion of Bub3 results in reduced levels of kinetochore-localized Ipl1 and concomitant massive chromosome missegregation caused by incorrect chromosome-spindle attachments. Depletion of Bub3 also results in shorter metaphase I and metaphase II due to premature localization of protein phosphatase 1 (PP1) to kinetochores, which antagonizes Ipl1-mediated phosphorylation. We propose a new role for the Bub1-Bub3 pathway in maintaining the balance between kinetochore localization of Ipl1 and PP1, a balance that is essential for accurate meiotic chromosome segregation and timely anaphase onset.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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Proteínas Serina-Treonina Quinasas
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Proteínas de Ciclo Celular
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Segregación Cromosómica
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Proteínas de Saccharomyces cerevisiae
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Meiosis
Idioma:
En
Revista:
J Cell Biol
Año:
2020
Tipo del documento:
Article
País de afiliación:
India