Enhancing data acquisition for the analysis of complex organic matter in direct-infusion Orbitrap mass spectrometry using micro-scans.
Rapid Commun Mass Spectrom
; 34(15): e8818, 2020 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-32342561
ABSTRACT
RATIONALE Acquisition quality in analytical science is key to obtaining optimal data from a sample. In very high-resolution mass spectrometry, quality is driven by the optimization of multiple parameters, including the use of scans and micro-scans (or transients) for performing a Fourier transformation. METHODS:
Thirty-nine mass spectra of a single synthesized complex sample were acquired using various numbers of scans and micro-scans determined through a simple experimental design. An electrospray ionization source coupled with an LTQ Orbitrap XL™ mass spectrometer was used, and acquisition was performed using a single mass range. All the resulting spectra were treated in the same way to enable comparisons of assigned stoichiometric formulae between acquisitions.RESULTS:
Converting the number of scans into micro-scans enhances signal quality by lowering noise and reducing artifacts. This modification also increases the number of attributed stoichiometric formulae for an equivalent acquisition time, giving access to a larger molecular diversity for the analyzed complex sample.CONCLUSIONS:
For complex samples, the use of long acquisition times leads to optimal data quality, and the use of micro-scans instead of scans-only maximizes the number of attributed stoichiometric formulae.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Rapid Commun Mass Spectrom
Año:
2020
Tipo del documento:
Article
País de afiliación:
Francia