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The Effect of a Fast-Releasing Hydrogen Sulfide Donor on Vascularization of Subcutaneous Scaffolds in Immunocompetent and Immunocompromised Mice.
Smink, Alexandra M; Najdahmadi, Avid; Alexander, Michael; Li, Shiri; Rodriquez, Samuel; van Goor, Harry; Hillebrands, Jan-Luuk; Botvinick, Elliot; Lakey, Jonathan R T; Vos, Paul de.
Afiliación
  • Smink AM; Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, 9713 GZ Groningen, The Netherlands.
  • Najdahmadi A; Department of Surgery, University of California Irvine, Orange, CA 92868, USA.
  • Alexander M; Department of Chemical Engineering and Materials Science, University of California Irvine, Irvine, CA 92617, USA.
  • Li S; Department of Surgery, University of California Irvine, Orange, CA 92868, USA.
  • Rodriquez S; Department of Surgery, University of California Irvine, Orange, CA 92868, USA.
  • van Goor H; Department of Surgery, University of California Irvine, Orange, CA 92868, USA.
  • Hillebrands JL; Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, 9713 GZ Groningen, The Netherlands.
  • Botvinick E; Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, 9713 GZ Groningen, The Netherlands.
  • Lakey JRT; Department of Chemical Engineering and Materials Science, University of California Irvine, Irvine, CA 92617, USA.
  • Vos P; Department of Surgery, University of California Irvine, Orange, CA 92868, USA.
Biomolecules ; 10(5)2020 05 06.
Article en En | MEDLINE | ID: mdl-32384680
ABSTRACT
Islet transplantation into subcutaneous polymer scaffolds has shown to successfully induce normoglycemia in type 1 diabetes models. Vascularization of these scaffolds is imperative for optimal control of glucose levels. We studied the effect of the vascular stimulator hydrogen sulfide (H2S) on the degree of vascularization of a scaffold and the role of the immune system in this process. Scaffolds were subcutaneously implanted in immunocompetent C57BL/6 and immunocompromised nude mice. Mice received twice-daily intraperitoneal injections of the fast-releasing H2S donor sodium hydrosulfide (NaHS, 25 or 50 µmol/kg) or saline for 28 days. After 63 days the vascular network was analyzed by histology and gene expression. Here we showed that the vascularization of a subcutaneous scaffold in nude mice was significantly impaired by H2S treatment. Both the CD31 gene and protein expression were reduced in these scaffolds compared to the saline-treated controls. In C57BL/6 mice, the opposite was found, the vascularization of the scaffold was significantly increased by H2S. The mRNA expression of the angiogenesis marker CD105 was significantly increased compared to the controls as well as the number of CD31 positive blood vessels. In conclusion, the immune system plays an important role in the H2S mediated effect on vascularization of subcutaneous scaffolds.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Huésped Inmunocomprometido / Neovascularización Fisiológica / Sulfuro de Hidrógeno Límite: Animals Idioma: En Revista: Biomolecules Año: 2020 Tipo del documento: Article País de afiliación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Huésped Inmunocomprometido / Neovascularización Fisiológica / Sulfuro de Hidrógeno Límite: Animals Idioma: En Revista: Biomolecules Año: 2020 Tipo del documento: Article País de afiliación: Países Bajos