Molecular cloning and characterization of type III polyketide synthase from Plumbago zeylanica.
J Asian Nat Prod Res
; 23(5): 478-490, 2021 May.
Article
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| MEDLINE
| ID: mdl-32400171
ABSTRACT
Two cDNAs encoding type Ш polyketide synthase (PKS1) and chalcone synthase (CHS, PKS2), were cloned from fresh leaves of Plumbago zeylanica L. (P. zeylanica). Their heterologous expression revealed that PKS1 catalyzed the formation of five α-pyrones from three to six acetate units by accepting acetyl-CoA and malonyl-CoA. In contrast, PKS2 catalyzed the formation of naringenin and bisnoryangonin by accepting p-coumaroyl-CoA and malonyl-CoA. Naringenin is thought to be involved in the biosynthesis of various bioactive flavonoids. PKS2 can be used to molecular breeding to enhance the production of these useful secondary metabolites via its overexpression.[Formula see text].
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plumbaginaceae
Idioma:
En
Revista:
J Asian Nat Prod Res
Asunto de la revista:
BOTANICA
/
QUIMICA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Japón