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Deformation and poration of giant unilamellar vesicles induced by anionic nanoparticles.
Karal, Mohammad Abu Sayem; Ahammed, Shareef; Levadny, Victor; Belaya, Marina; Ahamed, Md Kabir; Ahmed, Marzuk; Mahbub, Zaid Bin; Ullah, A K M Atique.
Afiliación
  • Karal MAS; Department of Physics, Bangladesh University of Engineering and Technology, Dhaka, 1000, Bangladesh. Electronic address: asayem221@phy.buet.ac.bd.
  • Ahammed S; Department of Physics, Bangladesh University of Engineering and Technology, Dhaka, 1000, Bangladesh.
  • Levadny V; Theoretical Problem Center of Physico-Chemical Pharmacology, Russian Academy of Sciences, Moscow, 117977, Russia.
  • Belaya M; Department of Mathematics of Russian State University for the Humanities, Moscow, GSP-3, 125993, Russia.
  • Ahamed MK; Department of Physics, Bangladesh University of Engineering and Technology, Dhaka, 1000, Bangladesh.
  • Ahmed M; Department of Physics, Bangladesh University of Engineering and Technology, Dhaka, 1000, Bangladesh.
  • Mahbub ZB; Department of Mathematics and Physics, North South University, Dhaka, 1229, Bangladesh.
  • Ullah AKMA; Analytical Chemistry Laboratory, Chemistry Division, Atomic Energy Centre, Bangladesh Atomic Energy Commission, Dhaka, 1000, Bangladesh.
Chem Phys Lipids ; 230: 104916, 2020 08.
Article en En | MEDLINE | ID: mdl-32407734
The interaction of anionic magnetite nanoparticles (MNPs) of size 18 nm with negatively charged giant unilamellar vesicles (GUVs) formed from a mixture of neutral dioleoylphosphatidylcholine (DOPC) and negatively charged dioleoylphosphatidylglycerol (DOPG) lipids has been investigated. It has been obtained that NPs induces the deformation of spherical GUVs. The reaction of other GUVs on NPs consists in the appearance of pores in their membranes. We focused the effect of electrostatics on the interaction of charged membranes with MNPs. To study the influence of the surface charge of GUVs on the processes under consideration, we varied the fraction of DOPG in the vesicles from 0 to 100%. We examined the influence of salt concentration in the range of 50-300 mM NaCl concentration. To describe the degree of deformation, a special parameter compactness was introduced. The pore formation in the membranes of GUVs was investigated by the leakage of sucrose. The compactness increases with time and also NPs concentration. The fraction of deformed GUVs increases with the increase of surface charge density of membranes as well as the decrease of salt concentration in buffer. The value of compactness for neutral membrane is 1.25 times higher than that of charged ones. The fraction of deformed GUVs become constant after 20 min, however it increases with NPs concentration. The time taken for stochastic pore formation is less for charged membrane than neutral one. The physical mechanism explaining the experimental results obtained in these investigations.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Liposomas Unilamelares / Nanopartículas Idioma: En Revista: Chem Phys Lipids Año: 2020 Tipo del documento: Article Pais de publicación: Irlanda

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Liposomas Unilamelares / Nanopartículas Idioma: En Revista: Chem Phys Lipids Año: 2020 Tipo del documento: Article Pais de publicación: Irlanda