DNA Condensation with a Boron-Containing Cationic Peptide for Modeling Boron Neutron Capture Therapy.
Radiat Phys Chem Oxf Engl 1993
; 1662020 Jan.
Article
en En
| MEDLINE
| ID: mdl-32454570
ABSTRACT
The amino acid derivative 4-borono-L-phenylalanine (BPA) has been used in the radiation medicine technique boron neutron capture therapy (BNCT). Here we have characterized its interaction with DNA when incorporated into a positively charged hexa-L-arginine peptide. This ligand binds strongly to DNA and induces its condensation, an effect which is attenuated at higher ionic strengths. The use of an additional tetra-L-arginine ligand enables the preparation of a DNA condensate in the presence of a negligible concentration of unbound boron. Under these conditions, Monte Carlo simulation indicates that >85% of energy deposition events resulting from thermal neutron irradiation derive from boron fission. The combination of experimental model systems and simulations that we describe here provides a valuable tool for accurate track structure modeling of the DNA damage produced by the high LET particles involved in BNCT.
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Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Radiat Phys Chem Oxf Engl 1993
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos