[Screening of reference genes based on real-time quantitative PCR analysis in Monochasma savatieri].
Zhongguo Zhong Yao Za Zhi
; 45(8): 1887-1892, 2020 Apr.
Article
en Zh
| MEDLINE
| ID: mdl-32489074
ABSTRACT
Monochasma savatieri belongs to Scrophulariaceae family. It is a facultative parasitic plant distributed in southern China that has been found in Jiangxi, Guangdong, and Fujian provinces. Previous studies on this plant have focused on its chemical constituents and pharmacological effects. The research foundation of molecular biology and functional genomics is still very in-depth, and there is no report on the reference genes. In this study, 6 reference gene(UBQ, GAPDH, AP-2, ACT, TUB and CYP) from transcriptome database, were selected and analyzed in M. savatieri.The experiment mainly involve two variables, soil moisture content and arbuscular mycorrhizal symbiosis(Rhizophagus irregularis). And the different combination of the factors result in a total of 6 treatments.Statistical tools, including GeNorm, NormFinder and Bestkeeper were utilized to assess the suitability of reference genes based on their stability rankings for different treatment. The result showed that the stability of 6 reference genes were significantly different. TUB and GAPDH were the most stable gene, while ACT was the most instable one. The results would provide reliable and optional available reference genes in gene expression analysis of M. savatieri, which has important application value.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genes de Plantas
/
Regulación de la Expresión Génica de las Plantas
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2020
Tipo del documento:
Article
País de afiliación:
China