LINC00473 functions as an oncogene and predicts poor prognosis in pancreatic cancer via the cAMP/ß-catenin axis.
Eur Rev Med Pharmacol Sci
; 24(10): 5345-5352, 2020 05.
Article
en En
| MEDLINE
| ID: mdl-32495868
ABSTRACT
OBJECTIVE:
To investigate the expression and function of LINC00463 in pancreatic cancer (PC), and to demonstrate the relationship between LINC00473 expression and clinical pathological characteristics and prognosis of PC. PATIENTS ANDMETHODS:
Expressions of LINC00473 in PC tissues and cell lines were detected using quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). LINC00473 siRNA was synthesized to knock down the LINC00473 expression in PANC-1 cells. Proliferation, invasion, and migration abilities of experimental cells were analyzed using cell counting kit-8 (CCK-8) assay and transwell assay, respectively. cAMP activity was detected and protein expression of ß-catenin was measured to explain the underlying mechanism of LINC00473 in PC. The prognosis and clinical pathological features of PC patients were illustrated.RESULTS:
LINC00473 was highly expressed in PC tissues and cells. Higher level of LINC00473 was relative with larger tumor size, worse tumor node metastasis (TNM) stage, worse tumor differentiation, higher rates of perineural invasion, and lymphatic invasion. Knockdown of LINC00473 significantly inhibited cell growth, invasion, and migration of PANC-1 cells. LINC00473 activated cAMP and then promoted the phosphorylation of ß-catenin to promote the progression of PC. Furthermore, high expression of LINC00473 and ß-catenin remarkedly indicated poor prognosis of PC patients.CONCLUSIONS:
LINC00473 was upregulated in PC tissues and cells, indicating a poor prognosis and clinical pathological features of PC. It promoted PC progression via activating the cAMP/ß-catenin axis, which provided a novel target for the prediction for PC diagnosis, biological therapy, and prognosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Pancreáticas
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AMP Cíclico
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Beta Catenina
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ARN Largo no Codificante
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
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Risk_factors_studies
Límite:
Aged
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Female
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Humans
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Male
Idioma:
En
Revista:
Eur Rev Med Pharmacol Sci
Asunto de la revista:
FARMACOLOGIA
/
TOXICOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
China