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[Dihydroartemisinin Induces Apoptosis of Human Acute T Lymphocytic Leukemia Cells by Activating Oxidative Stress].
Sun, Wei-Dong; Yu, Xing-Xing; An, Yi-Han; Wang, Xin; Wang, Ying; Tong, Xiang-Min.
Afiliación
  • Sun WD; Graduate School of Bengbu Medical College, Bengbu 233000, Anhui Province, China,Key Laboratory of Molecular Diagnosis and Individualization of Cancer, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang Province,China.
  • Yu XX; Graduate School of Bengbu Medical College, Bengbu 233000, Anhui Province, China,Key Laboratory of Molecular Diagnosis and Individualization of Cancer, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang Province,China.
  • An YH; Graduate School of Bengbu Medical College, Bengbu 233000, Anhui Province, China,Key Laboratory of Molecular Diagnosis and Individualization of Cancer, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang Province,China.
  • Wang X; Key Laboratory of Molecular Diagnosis and Individualization of Cancer, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang Province,China.
  • Wang Y; Key Laboratory of Molecular Diagnosis and Individualization of Cancer, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang Province,China.
  • Tong XM; Key Laboratory of Molecular Diagnosis and Individualization of Cancer, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang Province,China,E-mail: tongxiangmin11@163.com.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(3): 753-757, 2020 Jun.
Article en Zh | MEDLINE | ID: mdl-32552932
OBJECTIVE: To investigate the effects of dihydroartemisinin (DHA) on the proliferation and apoptosis of human T-cell acute lymphoblastic leukemia (T-ALL) Jurkat cell. METHODS: The effects of DHA on the proliferation of Jurkat cells and the recovery of DHA-inhibited cell viability by N-acetyl-L-cysteine (NAC) were examined by CCK-8 assay. Flow cytometry was performed to analyze the cell apoptosis and generation of reactive oxygen species (ROS). Western-blot was used to detected protein expression of DNA damage-related genes, as well as apoptosis-associated genes, respectively. RESULTS: DHA inhibited the proliferation of Jurkat cells, and shows a concentration-dependent manner(r =0.936), and NAC could partially restore the activity of DHA on cell proliferation inhibition. With the increase of drug concentration, the apoptosis rate (r =0.946) and ROS accumulation was increased (r =0.965). Western blot showed that the protein expressions of DNA damage-related gene γ-H2AX and apoptosis-related genes p53, c-Caspase3, BAX and cPARP were significantly increased, and BCL-2 protein expression was decreased. CONCLUSION: DHA can induce ROS production in Jurkat cells, which can cause DNA damage, activate the P53 apoptotic pathway, and promote apoptosis of cells.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia-Linfoma Linfoblástico de Células T Precursoras Límite: Humans Idioma: Zh Revista: Zhongguo Shi Yan Xue Ye Xue Za Zhi Asunto de la revista: HEMATOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia-Linfoma Linfoblástico de Células T Precursoras Límite: Humans Idioma: Zh Revista: Zhongguo Shi Yan Xue Ye Xue Za Zhi Asunto de la revista: HEMATOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: China