Comparison of five different laboratory techniques for the rabies diagnosis in clinically suspected cattle in Brazil.
J Virol Methods
; 283: 113918, 2020 09.
Article
en En
| MEDLINE
| ID: mdl-32554044
ABSTRACT
The direct-fluorescent antibody test (dFAT) is considered the "gold standard" assay to diagnose rabies. However, it is crucial to develop molecular techniques, such as RT-PCR and RT-qPCR, since many laboratories lack the needed supplies for performing complementary methods (viral isolation, for example). For this purpose, diagnostic techniques must be specific and sensitive to guarantee accuracy. This present investigation aimed to detect rabies virus (RABV) in 126 clinically suspected cattle in Brazil using different diagnostic tests [dFAT, mouse inoculation test (MIT), immunohistochemistry (IHC), RT-PCR and RT-qPCR] and to compare those results obtained under routine laboratory conditions. The results of the present investigation demonstrate that the molecular techniques are more sensitive and may detect low viral load, even though the non-homogeneous viral distribution caused a false-negative result in dFAT. We also observed a usual alteration in antigens distribution among regions of the central nervous system (CNS). By both dFAT and IHC assays, the most reliable CNS structures were thalamus and midbrain. Although this investigation demonstrated diagnostic sensitivity and specificity close to 100 % in all laboratory techniques employed, a dFAT auxiliary test is required for bovine specimens, such as molecular techniques, when there are poor sampling conditions (low viral load combined with unavailability of brainstem structures).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rabia
/
Pruebas Inmunológicas
/
Enfermedades de los Bovinos
/
Técnicas de Laboratorio Clínico
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
J Virol Methods
Año:
2020
Tipo del documento:
Article
País de afiliación:
Brasil