Recombinant ferritin nanoparticles can induce dendritic cell maturation through TLR4/NF-κB pathway.
Biotechnol Lett
; 42(12): 2489-2500, 2020 Dec.
Article
en En
| MEDLINE
| ID: mdl-32567013
ABSTRACT
OBJECTIVE:
Immune response initiation and regulation require activation of dendritic cells (DCs). However, the mechanism by which ferritin, a carrier for immunogen, induces DCs maturation remains unclear.RESULTS:
Recombinant ferritin nanoparticle (RFNp), were prepared through the baculovirus expression vector system, formed spherical and hollow cage-liked proteins with a diameter of approximately 12.17 ± 0.87 nm. They induced bone marrow-derived DC (BMDC) maturation via surface molecules up-regulation of (MHC II, CD80, CD86 and CD40), increased pro-inflammatory cytokines production (IL-6, IL-12, TNF-α, and IFN-γ), and decreased antigen capturing capacity. They positively regulated IκBα and NF-κB (p65) phosphorylation, and facilitate NF-κB (p65) translocation into mature BMDCs nuclei. Following pre-treatment of RFNp-treated BMDCs with TLR4 and NF-κB (p65) inhibitors, respectively, surface molecule expression, pro-inflammatory cytokines production, and IκBα and NF-κB (p65) activities were suppressed. RFNp-treated BMDCs can also facilitate T-cell proliferation and differentiation into Th1 and Th2.CONCLUSION:
RFNps induced DCs maturation lends the potential application of RFNps as carrier platforms in DC-based vaccine.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factor de Transcripción ReIA
/
Receptor Toll-Like 4
/
Nanopartículas
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Ferritinas
Límite:
Animals
Idioma:
En
Revista:
Biotechnol Lett
Año:
2020
Tipo del documento:
Article