Toxicological effects of chlorophenols to green algae observed at various pH and concentration of humic acid.

Humic acid (HA) is ubiquitous organic matter derived by microbial metabolisms. This polymeric substance has both hydrophilic and hydrophobic moieties, and it is known that they affect to bioavailability of environmental pollutants. Objective of this study is to investigate the toxicological effects of chlorophenols to green algae observed at various pH and concentration of HA. Toxicity was determined by algal growth inhibition rate and EC50 of green algae Chlorella vulgaris. As a result, toxicity of 2,4-dichlorophenol was mitigated with increase of the coexisting amount of HA and solution pH. In the case of coexisting 2.5 ppm HA, EC50 of 2,4-dichlorophenol was 12.2 ppm and approximately three times higher than the case of absence of HA at pH 7.5. Meanwhile, Toxicity of 2,4,6-trichlorophenol was enhanced with increase of the coexisting amount of HA. In the case of absence of HA, EC50 of 2,4,6-trichlorophenol was 13.1 ppm and approximately two times higher than the case of coexisting 2.5 ppm HA at pH 7.5. Results suggested that toxicity of chlorophenols is influenced by the electrostatic and hydrophobic interaction between HA and chlorophenols. The hypothesis of toxicity enhancement pathway was proposed in the case of equilibrium-state 2,4,6-trichlorophenol between anionic and nonionic states.