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Crocin Improves the Quality of Cryopreserved Goat Semen in Different Breeds.
Longobardi, Valentina; Zullo, Gianluigi; Cotticelli, Alessio; Salzano, Angela; Albero, Giuseppe; Navas, Luigi; Rufrano, Domenico; Claps, Salvatore; Neglia, Gianluca.
Afiliación
  • Longobardi V; Department of Veterinary Medicine and Animal Production, Federico II University, 80137 Naples, Italy.
  • Zullo G; Department of Precision Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, Italy.
  • Cotticelli A; Italian Buffalo Breeders Association, V. Petrarca 42/44, 81100 Caserta, Italy.
  • Salzano A; Department of Veterinary Medicine and Animal Production, Federico II University, 80137 Naples, Italy.
  • Albero G; Department of Veterinary Medicine and Animal Production, Federico II University, 80137 Naples, Italy.
  • Navas L; Department of Veterinary Medicine and Animal Production, Federico II University, 80137 Naples, Italy.
  • Rufrano D; Department of Veterinary Medicine and Animal Production, Federico II University, 80137 Naples, Italy.
  • Claps S; Council for Agricultural Research and Agricultural Economy Analysis-Research Centre for Animal Production and Aquaculture, S.S.7 Via Appia, 85051 Bella Muro, Italy.
  • Neglia G; Council for Agricultural Research and Agricultural Economy Analysis-Research Centre for Animal Production and Aquaculture, S.S.7 Via Appia, 85051 Bella Muro, Italy.
Animals (Basel) ; 10(6)2020 Jun 26.
Article en En | MEDLINE | ID: mdl-32604736
The effect of crocin in the semen extender before cryopreservation was evaluated on sperm parameters of 20 bucks of five different breeds: Garganica (GA), Jonica (JO), Maltese (MA), Mediterranean Red (MR) and Saanen (SA). Semen samples were centrifuged, to remove seminal plasma, divided in two aliquots and diluted with Tris-egg-yolk-based extender, containing 0 (control group) and 1 mM crocin. Crocin concentration was established after a preliminary dose trial. On fresh and frozen-thawed sperm, motility, viability, morphology, membrane integrity, DNA fragmentation and ROS levels were evaluated. The freezing process led to a decrease (p < 0.05) in all the sperm parameters recorded, confirming the deleterious effect of cryopreservation on goat semen. The most interesting result regarding the inclusion of crocin in the extender before cryopreservation was as follows: Crocin significantly improved (p < 0.05) sperm motility in all breeds, except for Mediterranean Red, compared to the control group. Furthermore, 1 mM crocin reduced percentage of spermatozoa with DNA fragmentation with a marked decrement (p < 0.05) in Garganica and Saanen, as compared to the control group. Finally, intracellular ROS decreased (p < 0.01) in the crocin-treated sperm of all breeds, as compared to the control. In conclusion, supplementation of 1 mM crocin in the extender decreased oxidative stress, improving sperm motility and the DNA integrity of frozen-thawed sperm in different breeds.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Animals (Basel) Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Animals (Basel) Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza