Genetic variability of grapevine fabavirus variants and development of a broad-spectrum assay for their detection.
Arch Virol
; 165(9): 2073-2078, 2020 Sep.
Article
en En
| MEDLINE
| ID: mdl-32621153
ABSTRACT
Complete RNA1 and RNA2 sequences of two and nearly complete genome sequences of six new variants of grapevine fabavirus found in Japan were compared to those of previously reported variants. Negative selection pressure was suggested, and no recombination events were detected in either RNA1 or RNA2. The first 18 nucleotides in both RNAs were predicted to form a stem-loop structure. The variants could be genetically divided into four groups based on RNA1 and two based on RNA2. A broad-spectrum reverse transcription polymerase chain reaction assay using a primer pair designed based on an RNA2 consensus sequence was able to detect all of the known variants.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de las Plantas
/
Variación Genética
/
Fabavirus
/
Vitis
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Arch Virol
Año:
2020
Tipo del documento:
Article
País de afiliación:
Japón