New 20(S)-protopanaxadiol type saponins from the leaves of Panax notoginseng and their potential anti-inflammatory activities.
Steroids
; 162: 108696, 2020 10.
Article
en En
| MEDLINE
| ID: mdl-32649999
ABSTRACT
Through the combination of various chromatographies, 11 new 20(S)-protopanaxadiol (PPD) type saponins, named as notoginsenosides NL-E1 - NL-E4 (1-4), NL-F1 (5), NL-F2 (6), NL-G1 (7), NL-G2 (8), NL-H1 - NL-H3 (9-11) were obtained from the leaves of Panax notoginseng. Their structures were ascertained based on the extensive spectroscopic methods and chemical reactions. Meanwhile, the 20(S)-PPD type saponins with aglycone, (20S,24ζ)-3ß,12ß,20,24,25-pentahydroxy dammarane, was only found from the leaves of P. notoginseng. The characteristic could be used to distinguish the extracts of P. notoginseng leaves from its other medicinal parts such as roots, rhizomes, flowers or seeds. Furthermore, the nitric oxide (NO) inhibitory activities of all compounds were examined in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. As a result, compounds 2-7, 10 could exert NO inhibitory activity at 25 µM without cytotoxicity. Moreover, the inhibitory activities of them were in dose-dependent manner at 1, 10, and 25 µM. Especially, notoginsenoside NL-F2 (6) still possessed strong biological activity at 1 µM.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sapogeninas
/
Hojas de la Planta
/
Panax notoginseng
/
Antiinflamatorios
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Steroids
Año:
2020
Tipo del documento:
Article
País de afiliación:
China