Cloning and Detection of Genomic Retrozymes and Their circRNA Intermediates.
Methods Mol Biol
; 2167: 27-44, 2021.
Article
en En
| MEDLINE
| ID: mdl-32712913
ABSTRACT
Retrozymes are a novel family of non-autonomous retrotransposable elements that contain hammerhead ribozyme motifs. These retroelements are found widespread in eukaryotic genomes, with active copies present in many species, which rely on other autonomous transposons for mobilization. Contrary to other retrotransposons, transcription of retrozymes in vivo leads to the formation and accumulation of circular RNAs, which can be readily detected by RNA blotting. In this chapter, we describe the procedures needed to carry out the cloning of genomic retrozymes, and to detect by northern blot their circular RNA retrotransposition intermediates.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Northern Blotting
/
ARN Catalítico
/
Clonación Molecular
/
Retroelementos
/
ARN Circular
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article
País de afiliación:
España