Detection of beta-lactamase production in clinical Prevotella species by MALDI-TOF MS method.
Anaerobe
; 65: 102240, 2020 Oct.
Article
en En
| MEDLINE
| ID: mdl-32768494
ABSTRACT
Penicillins, can be used in treatment of infections due to Prevotella species if they are susceptible to penicillin. Early and accurate preliminary detection of ß-lactamase-producing isolates is crucial for treatment of infection. The aim of this study was to determine ß-lactamase-producing Prevotella species by MALDI-TOF MS and screen them for the presence of cfxA gene, responsible for ß-lactamase production. A total of 500 clinically relevant Prevotella isolates, collected from 13 countries for the previous European antibiotic resistance surveillance study, were tested. Susceptibility testing was performed against ampicillin and ampicillin/sulbactam by Etest methodology. EUCAST guidelines were used for susceptibility interpretations; the isolates with MIC valueâ¯≤â¯0.5 for ampicillin were considered susceptible and >2 resistant. All Prevotella isolates, were tested for detection of ß-lactamase activity by MALDI-TOF MS (Vitek® MS Research Use Only) system and the presence of the cfxA gene by PCR method. The susceptibility levels of the isolates to ampicillin/sulbactam and ampicillin were 99.6% and 43.4%, respectively. A total 59% of isolates presented ß-lactamase activity and 60.8% were cfxA gene positive. Both these tests were positive for isolates in the resistant category. Additionally, >95% of the isolates (nâ¯=â¯65) which ampicillin MIC values ranged from >0.5⯵g/mL to 2⯵g/ml displayed ß-lactamase activity. We also found that the MALDI-TOF MS-based ß-lactamase assay delivers results in 2â¯h. We found a high concordance between the MALDI-TOF MS ß-lactamase results in terms of cfxA ß-lactamase gene presence. MALDI-TOF MS may serve as a simple and efficient alternative method of the existing phenotypic and PCR-based methods.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Beta-Lactamasas
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Técnicas de Tipificación Bacteriana
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Infecciones por Bacteroidaceae
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Prevotella
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Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
Tipo de estudio:
Clinical_trials
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Diagnostic_studies
/
Guideline
Límite:
Humans
Idioma:
En
Revista:
Anaerobe
Año:
2020
Tipo del documento:
Article