A device for stereotaxic viral delivery into the brains of neonatal mice.
Biotechniques
; 69(4): 307-312, 2020 10.
Article
en En
| MEDLINE
| ID: mdl-32777950
ABSTRACT
The increasing interest in manipulating neural circuits in developing brains has created a demand for reliable and accurate methods for delivering viruses to newborn mice. Here we describe a novel 3D-printed mouse neonatal stereotaxic adaptor for intracerebral viral injection that provides enhanced precision and reliability. Using this device, we injected A2a-Cre mice with a Cre-dependent hM4D-mCherry viral construct at postnatal day 1 (P1) and demonstrated selective expression in the striatal indirect pathway neurons on days P7, P11 and P25. Similarly, dopaminergic midbrain neurons were selectively targeted with a Cre-dependent green fluorescent protein virus in Dat-IRES-Cre neonates and expression examined at P25. Our open-source neonatal stereotaxic mouse adaptor facilitates neonatal neuronal targeting, which should improve the ability to label and modify neural circuits in developing mouse brains.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus
/
Encéfalo
/
Técnicas Estereotáxicas
/
Técnicas de Transferencia de Gen
Límite:
Animals
Idioma:
En
Revista:
Biotechniques
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos