Real-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric Micelles.
Nano Lett
; 20(9): 6466-6472, 2020 09 09.
Article
en En
| MEDLINE
| ID: mdl-32787172
ABSTRACT
Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln3+-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to â¼20 degrees and subcellular gradients of â¼5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Elementos de la Serie de los Lantanoides
Idioma:
En
Revista:
Nano Lett
Año:
2020
Tipo del documento:
Article
País de afiliación:
España