Exosomes derived from HEK293T cells interact in an efficient and noninvasive manner with mammalian sperm in vitro.
Nanomedicine (Lond)
; 15(20): 1965-1980, 2020 08.
Article
en En
| MEDLINE
| ID: mdl-32794431
ABSTRACT
Aim:
To investigate exosomes as a noninvasive delivery tool for mammalian sperm. Materials &Methods:
Exosomes were isolated from HEK293T cells and co-incubated with boar sperm in vitro.Results:
Internalized exosomes were detected within 10 min of co-incubation. Computer-assisted sperm analysis and flow cytometry demonstrated that even after 5-h of exposure to exosomes, there were no significant deleterious effects with regard to sperm motility, viability, membrane integrity and mitochondrial membrane potential (p > 0.05), thus indicating that exosomes did not interfere with basic sperm function.Conclusion:
HEK293T-derived exosomes interacted with boar sperm without affecting sperm function. Exosomes represent a versatile and promising research tool for studying sperm biology and provide new options for the diagnosis and treatment of male infertility.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Motilidad Espermática
/
Exosomas
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Nanomedicine (Lond)
Año:
2020
Tipo del documento:
Article
País de afiliación:
Reino Unido