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A class I histone deacetylase is implicated in the encystation of Entamoeba invadens.
Lozano-Amado, Daniela; Ávila-López, Pedro Antonio; Hernández-Montes, Georgina; Briseño-Díaz, Paola; Vargas, Miguel; Lopez-Rubio, Jose-Juan; Carrero, Julio César; Hernández-Rivas, Rosaura.
Afiliación
  • Lozano-Amado D; Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México, Mexico.
  • Ávila-López PA; Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México, Mexico.
  • Hernández-Montes G; Coordinación de la Investigación Científica, Red de Apoyo a la Investigación, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México, Mexico.
  • Briseño-Díaz P; Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México, Mexico.
  • Vargas M; Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México, Mexico.
  • Lopez-Rubio JJ; LPHI - Laboratory of Pathogen Host Interactions - UMR5235, CNRS, INSERM, Université de Montpellier, Montpellier, France.
  • Carrero JC; Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México, Mexico.
  • Hernández-Rivas R; Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México, Mexico. Electronic address: rohernan@cinvestav.mx.
Int J Parasitol ; 50(12): 1011-1022, 2020 10.
Article en En | MEDLINE | ID: mdl-32822677
ABSTRACT
Epigenetic mechanisms such as histone acetylation and deacetylation participate in regulation of the genes involved in encystation of Entamoeba invadens. However, the histones and target residues involved, and whether the acetylation and deacetylation of the histones leads to the regulation of gene expression associated with the encystation of this parasite, remain unknown. In this study, we found that E. invadens histone H4 is acetylated in both stages of the parasite and is more highly acetylated during the trophozoite stage than in the cyst. Histone hyperacetylation induced by Trichostatin A negatively affects the encystation of E. invadens, and this inhibition is associated with the downregulation of the expression of genes implicated in the synthesis of chitin, polyamines, gamma-aminobutyric acid pathways and cyst wall proteins, all of which are important in the formation of cysts. Finally, in silico analysis and activity assays suggest that a class I histone deacetylase (EiHDAC3) could be involved in control of the expression of a subset of genes that are important in several pathways during encystation. Therefore, the identification of enzymes that acetylate and/or deacetylate histones that control encystation in E. invadens could be a promising therapeutic target for preventing transmission of other amoebic parasites such as E. histolytica, the causative agent of amoebiasis in humans.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Entamoeba / Histona Desacetilasas Límite: Animals / Humans Idioma: En Revista: Int J Parasitol Año: 2020 Tipo del documento: Article País de afiliación: México

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Entamoeba / Histona Desacetilasas Límite: Animals / Humans Idioma: En Revista: Int J Parasitol Año: 2020 Tipo del documento: Article País de afiliación: México