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Improved reference genome of the arboviral vector Aedes albopictus.
Palatini, Umberto; Masri, Reem A; Cosme, Luciano V; Koren, Sergey; Thibaud-Nissen, Françoise; Biedler, James K; Krsticevic, Flavia; Johnston, J Spencer; Halbach, Rebecca; Crawford, Jacob E; Antoshechkin, Igor; Failloux, Anna-Bella; Pischedda, Elisa; Marconcini, Michele; Ghurye, Jay; Rhie, Arang; Sharma, Atashi; Karagodin, Dmitry A; Jenrette, Jeremy; Gamez, Stephanie; Miesen, Pascal; Masterson, Patrick; Caccone, Adalgisa; Sharakhova, Maria V; Tu, Zhijian; Papathanos, Philippos A; Van Rij, Ronald P; Akbari, Omar S; Powell, Jeffrey; Phillippy, Adam M; Bonizzoni, Mariangela.
Afiliación
  • Palatini U; Department of Biology and Biotechnology, University of Pavia, Pavia, 27100, Italy.
  • Masri RA; Department of Entomology and the Fralin Life Science Institute, Virginia Polytechnic and State University, Blacksburg, VA, 24061, USA.
  • Cosme LV; Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT, 06511-8934, USA.
  • Koren S; Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, 20892-2152, MD, USA.
  • Thibaud-Nissen F; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, 20894, MD, USA.
  • Biedler JK; Department of Entomology and the Fralin Life Science Institute, Virginia Polytechnic and State University, Blacksburg, VA, 24061, USA.
  • Krsticevic F; Department of Entomology, Robert H Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 7610001, Rehovot, Israel.
  • Johnston JS; Department of Entomology, Texas A&M University, College Station, TX, 77843, USA.
  • Halbach R; Department of Medical Microbiology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands.
  • Crawford JE; Verily Life Sciences, South San Francisco, 94080, CA, USA.
  • Antoshechkin I; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
  • Failloux AB; Department of Virology, Arbovirus and Insect Vectors Units, Institut Pasteur, Paris, 75015, France.
  • Pischedda E; Department of Biology and Biotechnology, University of Pavia, Pavia, 27100, Italy.
  • Marconcini M; Department of Biology and Biotechnology, University of Pavia, Pavia, 27100, Italy.
  • Ghurye J; Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, 20892-2152, MD, USA.
  • Rhie A; Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, 20892-2152, MD, USA.
  • Sharma A; Department of Entomology and the Fralin Life Science Institute, Virginia Polytechnic and State University, Blacksburg, VA, 24061, USA.
  • Karagodin DA; Laboratory of Evolutionary Genomics of Insects, The Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
  • Jenrette J; Department of Entomology and the Fralin Life Science Institute, Virginia Polytechnic and State University, Blacksburg, VA, 24061, USA.
  • Gamez S; Division of Biological Sciences, University of California, San Diego, La Jolla, CA, 92093-0349, USA.
  • Miesen P; Department of Medical Microbiology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands.
  • Masterson P; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, 20894, MD, USA.
  • Caccone A; Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT, 06511-8934, USA.
  • Sharakhova MV; Department of Entomology and the Fralin Life Science Institute, Virginia Polytechnic and State University, Blacksburg, VA, 24061, USA.
  • Tu Z; Laboratory of Evolutionary Genomics of Insects, The Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
  • Papathanos PA; Laboratory of Ecology, Genetics and Environment Protection, Tomsk State University, Tomsk, 634041, Russia.
  • Van Rij RP; Department of Entomology and the Fralin Life Science Institute, Virginia Polytechnic and State University, Blacksburg, VA, 24061, USA.
  • Akbari OS; Department of Entomology, Robert H Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 7610001, Rehovot, Israel.
  • Powell J; Department of Medical Microbiology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands.
  • Phillippy AM; Division of Biological Sciences, University of California, San Diego, La Jolla, CA, 92093-0349, USA.
  • Bonizzoni M; Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT, 06511-8934, USA.
Genome Biol ; 21(1): 215, 2020 08 26.
Article en En | MEDLINE | ID: mdl-32847630
ABSTRACT

BACKGROUND:

The Asian tiger mosquito Aedes albopictus is globally expanding and has become the main vector for human arboviruses in Europe. With limited antiviral drugs and vaccines available, vector control is the primary approach to prevent mosquito-borne diseases. A reliable and accurate DNA sequence of the Ae. albopictus genome is essential to develop new approaches that involve genetic manipulation of mosquitoes.

RESULTS:

We use long-read sequencing methods and modern scaffolding techniques (PacBio, 10X, and Hi-C) to produce AalbF2, a dramatically improved assembly of the Ae. albopictus genome. AalbF2 reveals widespread viral insertions, novel microRNAs and piRNA clusters, the sex-determining locus, and new immunity genes, and enables genome-wide studies of geographically diverse Ae. albopictus populations and analyses of the developmental and stage-dependent network of expression data. Additionally, we build the first physical map for this species with 75% of the assembled genome anchored to the chromosomes.

CONCLUSION:

The AalbF2 genome assembly represents the most up-to-date collective knowledge of the Ae. albopictus genome. These resources represent a foundation to improve understanding of the adaptation potential and the epidemiological relevance of this species and foster the development of innovative control measures.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arbovirus / Genoma / Aedes / Mosquitos Vectores Límite: Animals Idioma: En Revista: Genome Biol Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2020 Tipo del documento: Article País de afiliación: Italia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arbovirus / Genoma / Aedes / Mosquitos Vectores Límite: Animals Idioma: En Revista: Genome Biol Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2020 Tipo del documento: Article País de afiliación: Italia