Catalytic properties and stability of three common variants of placental alkaline phosphatase.
Biochem Genet
; 16(5-6): 433-42, 1978 Jun.
Article
en En
| MEDLINE
| ID: mdl-32872
ABSTRACT
Three placental alkaline phosphatases purified to homogeneity, i.e., the F, I, and S variants, were investigated for catalytic and stability properties. All three forms of the enzyme were found to have almost identical pH optima (10.7--10.8), similar sensitivity to the uncompetitive inhibitors L-phenylalanine (70%) and L-leucine (30%), and identical Km values against p-nitrophenylphosphate, beta-glycerophosphate, and alpha-naphthylphosphate. Significant differences among the three types were observed in thermal stability. The F variant was found to be most stable and the I variant most labile at 79 C. At 70 C all three forms were stable.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Placenta
/
Variación Genética
/
Fosfatasa Alcalina
Límite:
Female
/
Humans
Idioma:
En
Revista:
Biochem Genet
Año:
1978
Tipo del documento:
Article