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Cell swelling, softening and invasion in a three-dimensional breast cancer model.
Han, Yu Long; Pegoraro, Adrian F; Li, Hui; Li, Kaifu; Yuan, Yuan; Xu, Guoqiang; Gu, Zichen; Sun, Jiawei; Hao, Yukun; Gupta, Satish Kumar; Li, Yiwei; Tang, Wenhui; Tang, Xiao; Teng, Lianghong; Fredberg, Jeffrey J; Guo, Ming.
Afiliación
  • Han YL; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Pegoraro AF; Department of Physics, University of Ottawa, Ottawa, ON, K1N 6N5, Canada.
  • Li H; Harvard John A. Paulson School of Engineering and Applied Sciences, Cambridge, MA, 02138, USA.
  • Li K; School of Systems Science, Beijing Normal University, Beijing 100875, P.R. China.
  • Yuan Y; Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, P.R. China.
  • Xu G; Xuanwu Hospital, Capital Medical University, Beijing, 100053, P.R. China.
  • Gu Z; Harvard John A. Paulson School of Engineering and Applied Sciences, Cambridge, MA, 02138, USA.
  • Sun J; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Hao Y; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Gupta SK; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Li Y; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Tang W; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Tang X; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Teng L; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
  • Fredberg JJ; Xuanwu Hospital, Capital Medical University, Beijing, 100053, P.R. China.
  • Guo M; Xuanwu Hospital, Capital Medical University, Beijing, 100053, P.R. China.
Nat Phys ; 16(1): 101-108, 2020 Jan.
Article en En | MEDLINE | ID: mdl-32905405
Sculpting of structure and function of three-dimensional multicellular tissues depend critically on the spatial and temporal coordination of cellular physical properties, yet the organizational principles that govern these events, and their disruption in disease, remain poorly understood. Using a multicellular mammary cancer organoid model, here we map in three dimensions the spatial and temporal evolution of positions, motions, and physical characteristics of individual cells. Compared with cells in the organoid core, cells at the organoid periphery and the invasive front are found to be systematically softer, larger and more dynamic. These mechanical changes are shown to arise from supracellular fluid flow through gap junctions, suppression of which delays transition to an invasive phenotype. Together, these findings highlight the role of spatiotemporal coordination of cellular physical properties in tissue organization and disease progression.

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Nat Phys Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Nat Phys Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido