Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore.
Methods Mol Biol
; 2186: 77-94, 2021.
Article
en En
| MEDLINE
| ID: mdl-32918731
ABSTRACT
Nanopore sensing is a powerful lab-on-a-chip technique that allows for the analysis of biomarkers present in small sample sizes. In general, nanopore clogging and low detection accuracy arise when the sample becomes more and more complex such as in blood or lysate. To address this, we developed an OmpG nanopore that distinguishes among not only different proteins in a mixture but also protein homologs. Here, we describe this OmpG-based nanopore system that specifically analyzes targets biomarkers in complex mixtures.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de la Membrana Bacteriana Externa
/
Biomarcadores
/
Proteínas
/
Porinas
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Proteínas de Escherichia coli
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Escherichia coli
/
Nanoporos
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos