Endophytic bacteria of garlic roots promote growth of micropropagated meristems.
Microbiol Res
; 241: 126585, 2020 Dec.
Article
en En
| MEDLINE
| ID: mdl-32919224
ABSTRACT
Garlic (Allium sativum L.) is one of the crops whose economic importance has increased considerably in recent years in Brazil. The use of plant growth-promoting bacteria (PGPB) is a useful alternative for reducing the cost of agricultural inputs and, consequently, for increasing productivity. Therefore, the main objective of this work was to isolate and evaluate potential growth promoters in plants and plant pathogenic fungi growth inhibitors using endophytic bacteria from garlic roots and bacteria from the Agricultural Microbiology Culture Collection at the Federal University of Lavras. Besides verifying improvements in the growth and physiology of garlic meristems grown in vitro under the action of PGPB. Forty-eight endophytic bacteria were identified using matrix-assisted laser desorption/ionization-time of flight mass spectrometry based on the protein profile of each isolate. Four isolates were chosen according to their ability to fix nitrogen, to produce auxin and solubilize phosphate. The cultivation of garlic meristems in tissue culture with these bacteria was established at a population level of 106 CFU/mL. The evaluated criteria were (1) the colonization capacity of the bacteria inside the garlic plants determined through scanning electron microscopy; (2) the chlorophyll content; and (3) the growth of garlic plants in vitro post-PGPB inoculation. Volatiles emitted by those isolates inhibited fungi growth. The inoculation of garlic meristems with Enterobacter cloacae and Burkholderia cepacia promoted an improvement in the growth and physiological attributes of garlic, indicating the feasibility of their application as plant growth promoters for commercial cultivation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacterias
/
Raíces de Plantas
/
Meristema
/
Desarrollo de la Planta
/
Ajo
Tipo de estudio:
Prognostic_studies
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Microbiol Res
Asunto de la revista:
MICROBIOLOGIA
/
SAUDE AMBIENTAL
Año:
2020
Tipo del documento:
Article