Paper-based fluorogenic RNA aptamer sensors for label-free detection of small molecules.
Anal Methods
; 12(21): 2674-2681, 2020 06 04.
Article
en En
| MEDLINE
| ID: mdl-32930298
ABSTRACT
Sensors based on fluorogenic RNA aptamers have emerged in recent years. These sensors have been used for in vitro and intracellular detection of a broad range of biological and medical targets. However, the potential application of fluorogenic RNA-based sensors for point-of-care testing is still little studied. Here, we report a paper substrate-based portable fluorogenic RNA sensor system. Target detection can be simply performed by rehydration of RNA sensor-embedded filter papers. This affordable sensor system can be used for the selective, sensitive, and rapid detection of different target analytes, such as antibiotics and cellular signaling molecules. We believe that these paper-based fluorogenic RNA sensors show great potential for point-of-care testing of a wide range of targets from small molecules, nucleic acids, proteins, to various pathogens.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Aptámeros de Nucleótidos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Methods
Año:
2020
Tipo del documento:
Article