Bioreactor-Based Antigen Production Process Using the Baculovirus Expression Vector System.
Methods Mol Biol
; 2183: 95-118, 2021.
Article
en En
| MEDLINE
| ID: mdl-32959243
ABSTRACT
Several vaccines are already produced using the baculovirus expression vector system (BEVS). This chapter describes methods for generating recombinant baculoviral DNA (also called bacmid) for cultivating Spodoptera frugiperda Sf-9 cells and producing a baculovirus stock from the recombinant bacmid and for producing a protein-based vaccine with the BEVS in a stirred tank reactor.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Recombinantes
/
Baculoviridae
/
Reactores Biológicos
/
Técnicas de Cultivo Celular por Lotes
/
Vectores Genéticos
/
Antígenos
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article
País de afiliación:
Alemania