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Defining the function of OmpA in the Rcs stress response.
Dekoninck, Kilian; Létoquart, Juliette; Laguri, Cédric; Demange, Pascal; Bevernaegie, Robin; Simorre, Jean-Pierre; Dehu, Olivia; Iorga, Bogdan I; Elias, Benjamin; Cho, Seung-Hyun; Collet, Jean-Francois.
Afiliación
  • Dekoninck K; WELBIO, Brussels, Belgium.
  • Létoquart J; de Duve Institute, Université catholique de Louvain (UCLouvain), Brussels, Belgium.
  • Laguri C; WELBIO, Brussels, Belgium.
  • Demange P; de Duve Institute, Université catholique de Louvain (UCLouvain), Brussels, Belgium.
  • Bevernaegie R; Univ. Grenoble Alpes, CNRS, CEA, Grenoble, France.
  • Simorre JP; Institut de Pharmacologie et de Biologie Structurale, IPBS, Université de Toulouse, CNRS, Université Paul Sabatier, Toulouse, France.
  • Dehu O; Institut de la Matière Condensée et des Nanosciences (IMCN), Molecular Chemistry, Materials and Catalysis (MOST), Université catholique de Louvain (UCLouvain), Louvain-la-Neuve, Belgium.
  • Iorga BI; Univ. Grenoble Alpes, CNRS, CEA, Grenoble, France.
  • Elias B; de Duve Institute, Université catholique de Louvain (UCLouvain), Brussels, Belgium.
  • Cho SH; de Duve Institute, Université catholique de Louvain (UCLouvain), Brussels, Belgium.
  • Collet JF; Université Paris-Saclay, CNRS UPR 2301, Institut de Chimie des Substances Naturelles, Gif-sur-Yvette, France.
Elife ; 92020 09 28.
Article en En | MEDLINE | ID: mdl-32985973
OmpA, a protein commonly found in the outer membrane of Gram-negative bacteria, has served as a paradigm for the study of ß-barrel proteins for several decades. In Escherichia coli, OmpA was previously reported to form complexes with RcsF, a surface-exposed lipoprotein that triggers the Rcs stress response when damage occurs in the outer membrane and the peptidoglycan. How OmpA interacts with RcsF and whether this interaction allows RcsF to reach the surface has remained unclear. Here, we integrated in vivo and in vitro approaches to establish that RcsF interacts with the C-terminal, periplasmic domain of OmpA, not with the N-terminal ß-barrel, thus implying that RcsF does not reach the bacterial surface via OmpA. Our results suggest a novel function for OmpA in the cell envelope: OmpA competes with the inner membrane protein IgaA, the downstream Rcs component, for RcsF binding across the periplasm, thereby regulating the Rcs response.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de la Membrana Bacteriana Externa / Transducción de Señal / Membrana Celular / Proteínas de Escherichia coli / Escherichia coli Idioma: En Revista: Elife Año: 2020 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de la Membrana Bacteriana Externa / Transducción de Señal / Membrana Celular / Proteínas de Escherichia coli / Escherichia coli Idioma: En Revista: Elife Año: 2020 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido