Isolation and Identification of Pathogenic Acanthamoeba Species from Air Conditioning Systems, Egypt.
Jpn J Infect Dis
; 74(3): 180-186, 2021 May 24.
Article
en En
| MEDLINE
| ID: mdl-32999181
ABSTRACT
Acanthamoeba are free-living amoebae that cause granulomatous amoebic encephalitis and keratitis. In this study, we aimed to isolate and identify Acanthamoeba from air conditioning systems using in vitro cell culture and polymerase chain reaction assays. We also estimated the pathogenicity of the isolates by measuring their thermotolerance and studying mice models inoculated with these isolates. Of the 80 dust samples acquired, 41 (51.25%) were found to be positive for Acanthamoeba spp. using in vitro cell culture and the results were validated using PCR. Out of these 41 samples, 27 (65.9%) were thermotolerant and 16 (39%) samples could infect mice and cause histopathological effects. Highly pathogenic Acanthamoeba isolates were characterized by their thermotolerance and the ability to disseminate in all organs after infection, causing early death of infected animals. Our study thus validated the presence of pathogenic isolates of Acanthamoeba in air conditioners that may be potentially infectious to humans.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Acanthamoeba
/
Aire Acondicionado
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
País/Región como asunto:
Africa
Idioma:
En
Revista:
Jpn J Infect Dis
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
Año:
2021
Tipo del documento:
Article
País de afiliación:
Egipto