An optimized step-by-step protocol for isolation of nucleus pulposus, annulus fibrosus, and end plate cells from the mouse intervertebral discs and subsequent preparation of high-quality intact total RNA.

Piprode, Vikrant; Mohanty, Sarthak; Bonavita, Raffaella; Loh, Sarah; Anbazhagan, Rajakumar; Saini, Chandan; Pinelli, Robert; Pricop, Paul; Dahia, Chitra L

Piprode, Vikrant; Mohanty, Sarthak; Bonavita, Raffaella; Loh, Sarah; Anbazhagan, Rajakumar; Saini, Chandan; Pinelli, Robert; Pricop, Paul; Dahia, Chitra L.

Afiliación

Piprode V; Hospital for Special Surgery New York New York USA.
Mohanty S; Hospital for Special Surgery New York New York USA.
Bonavita R; Hospital for Special Surgery New York New York USA.
Loh S; Hospital for Special Surgery New York New York USA.
Anbazhagan R; Hospital for Special Surgery New York New York USA.
Saini C; Hospital for Special Surgery New York New York USA.
Pinelli R; Hospital for Special Surgery New York New York USA.
Pricop P; Hospital for Special Surgery New York New York USA.
Dahia CL; Hospital for Special Surgery New York New York USA.

JOR Spine ; 3(3): e1108, 2020 Sep.

Article en En | MEDLINE | ID: mdl-33015579

Palabras clave

Preclinical models; development; genetics; regenerative medicine

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: JOR Spine Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos

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