Pathogenic Detection by Metagenomic Next-Generation Sequencing in Osteoarticular Infections.
Front Cell Infect Microbiol
; 10: 471, 2020.
Article
en En
| MEDLINE
| ID: mdl-33042860
ABSTRACT
Objectives:
To evaluate metagenomic next-generation sequencing (mNGS) as a diagnostic tool in detecting pathogens from osteoarticular infection (OAI) samples.Methods:
130 samples of joint fluid, sonicate fluid, and tissue were prospectively collected from 92 patients with OAI. The performance of mNGS and microbiology culture was compared pairwise.Results:
The overall sensitivity of mNGS was 88.5% (115/130), significantly higher than that of microbiological culture, which had a sensitivity of 69.2% (90/130, p < 0.01). Sensitivity was significantly higher for joint fluid (mNGS 86.7% vs. microbiology culture 68.7%, p < 0.01) and sonicate fluid (mNGS 100% vs. microbiology culture 66.7%, p < 0.05) samples. mNGS detected 12 pathogenic strains undetected by microbiological culture. Additional pathogens detected by mNGS were Coagulase-negative Staphylococci, Gram-negative Bacillus, Streptococci, Anaerobe, non-tuberculosis mycobacterium, MTCP (p > 0.05), and Mycoplasma (OR = ∞, 95% confidence interval, 5.12-∞, p < 0.001). Additionally, sensitivity by mNGS was higher in antibiotic-treated samples compared to microbiological culture (89.7 vs. 61.5%, p < 0.01).Conclusions:
mNGS is a robust diagnostic tool for pathogenic detection in samples from OAI patients, compared to routine cultures. The mNGS technique is particularly valuable to diagnose pathogens that are difficult to be cultured, or to test samples from patients previously treated with antibiotics.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Metagenoma
/
Metagenómica
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Front Cell Infect Microbiol
Año:
2020
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
CH
/
SUIZA
/
SUÍÇA
/
SWITZERLAND