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Phylogenetic analysis and ontogenetic changes in the cone opsins of the western mosquitofish (Gambusia affinis).
Chang, Chia-Hao; Wang, Yu-Chun; Shao, Yi Ta; Liu, Shih-Hui.
Afiliación
  • Chang CH; TIGP Biodiversity Program, Tunghai University, Taichung City, Taiwan.
  • Wang YC; Center for Ecology and Environment, Tunghai University, Taichung City, Taiwan.
  • Shao YT; Planning and Information Division, Fisheries Research Institute, Keelung City, Taiwan.
  • Liu SH; Institute of Marine Biology, National Taiwan Ocean University, Keelung, Taiwan.
PLoS One ; 15(10): e0240313, 2020.
Article en En | MEDLINE | ID: mdl-33048954
To convert external light into internal neural signal, vertebrates rely on a special group of proteins, the visual opsins. Four of the five types of visual opsins-short-wavelength sensitive 1 (Sws1), short-wavelength sensitive 2 (Sws2), medium-wavelength sensitive (Rh2), and long-wavelength sensitive (Lws)-are expressed in cone cells for scotopic vision, with the fifth, rhodopsin (Rh1), being expressed in rod cells for photopic vision. Fish often display differing ontogenetic cone opsin expression profiles, which may be related to dietary and/or habitat ontogenetic shift. The western mosquitofish (Gambusia affinis) is an aggressive invader that has successfully colonized every continent except Antarctica. The strong invasiveness of this species may be linked to its visual acuity since it can inhabit turbid waters better than other fishes. By genome screening and transcriptome analysis, we identify seven cone opsin genes in the western mosquitofish, including one sws1, two sws2, one rh2, and three lws. The predicted maximal absorbance wavelength (λmax) values of the respective proteins are 353 nm for Sws1, 449 nm for Sws2a, 408 nm for Sws2b, 516 nm for Rh2-1, 571 nm for Lws-1, and 519 nm for Lws-3. Retention of an intron in the lws-r transcript likely renders this visual opsin gene non-functional. Our real-time quantitative PCR demonstrates that adult male and female western mosquitofish do not differ in their cone opsin expression profiles, but we do reveal an ontogenetic shift in cone opsin expression. Compared to adults, larvae express proportionally more sws1 and less lws-1, suggesting that the western mosquitofish is more sensitive to shorter wavelengths in the larval stage, but becomes more sensitive to longer wavelengths in adulthood.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ciprinodontiformes / Perfilación de la Expresión Génica / Opsinas de los Conos / Secuenciación Completa del Genoma Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2020 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ciprinodontiformes / Perfilación de la Expresión Génica / Opsinas de los Conos / Secuenciación Completa del Genoma Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2020 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos