AIEgen-Lipid Conjugate for Rapid Labeling of Neutrophils and Monitoring of Their Behavior.
Angew Chem Int Ed Engl
; 60(6): 3175-3181, 2021 02 08.
Article
en En
| MEDLINE
| ID: mdl-33084214
ABSTRACT
Studies on neutrophil-based nanotherapeutic engineering have shown great potentials in treating infection and inflammation disorders. Conventional neutrophil labeling methods are time-consuming and often result in undesired contamination and activation since neutrophils are terminal-differentiated cells with a half-life span of only 7â
h. A simple, fast, and biocompatible strategy to construct engineered neutrophils is highly desirable but remains difficult to achieve. In this study, we present an AIEgen-lipid conjugate, which can efficiently label harvested neutrophils in 30â
s with no washing step required. This fast labeling method does not affect the activation and transmigration property of neutrophils, which has been successfully used to monitor neutrophil behaviors such as the chemotaxis process and migrating function towards inflammation sites both inâ
vitro and inâ
vivo, offering a tantalizing prospect for neutrophil-based nanotherapeutics studies.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Lípidos
/
Neutrófilos
Límite:
Animals
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Año:
2021
Tipo del documento:
Article
País de afiliación:
Singapur